Consulta: creatorFacets:"Inoue, Noboru"
Registros recuperados: 36
Data/hora: 03/06/2024 12:16:53

A PCR-based survey of animal African trypanosomosis and selected piroplasm parasites of cattle and goats in Zambia
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Musinguzi, Simon Peter; Suganuma, Keisuke; Asada, Masahito; Laohasinnarong, Dusit; Sivakumar, Thillaiampalam; Yokoyama, Naoaki; Namangala, Boniface; Sugimoto, Chihiro; Suzuki, Yasuhiko; Xuan, Xuenan; Inoue, Noboru.
We screened cattle and goats from the districts of Chama, Monze and Mumbwa in Zambia for animal African trypanosomes, Babesia bigemina and Theileria parva using PCRs; 38.1% of the samples tested positive for at least one of the parasite species. The most common parasite was Trypanosoma vivax (19.8%). Its incidence was significantly higher in goats than in cattle, (P&lt;0.05). B. bigemina was found in samples from all the three areas, making it the most widespread of the parasites in Zambia. Among the tested samples, 12.0% of the positive samples were mixed infections. There were significant differences in the infection rates of T. vivax (Mumbwa had a significantly higher infection rate [39.6%, P&lt;0.0001]), Th. parva (Monze had the only cases...
Palavras-chave: Animal African trypanosomosis; Cattle; Goat; Piroplasmosis; Zambia.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4389

A trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks in Kagoshima Prefecture, Japan.
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Thekisoe, Oriel M. M.; Honda, T; Fujita, H; Battsetseg, B; Hatta, T; Fujisaki, Kozo; Sugimoto, Chihiro; Inoue, Noboru.
Common arthropod vectors for trypanosomes are flies, fleas and bugs. This study reports on an unknown trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks, hereby, referred to as Trypanosoma KG1 isolate. The parasite has been successfully cultured in vitro with L929 or HEK 293T cell line as feeder cells. This trypanosome cannot survive in vitro without feeder cells. Following experimental infections of ticks, the trypomastigote-like and the epimastigote-like forms of this trypanosome could be detected by Giemsa-stained smears of the midgut and salivary glands of Ornithodoros moubata ticks which were made to feed on a culturing medium containing Trypanosoma KG1 isolate through an artificial membrane. Trypanosoma KG1 isolate...
Palavras-chave: Trypanosoma KG1 isolate; Haemaphysalis hystricis; Ornithodoros moubata.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1047

Adjuvant Effect of Oligomannose-Coated Liposome-Based Platform for veccine against African Trypanosomosis
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Kuboki, Noritaka; Yokoyama, Naoaki; Namangala, B; Okamura, Masashi; Inoue, Noboru; Takagi, Hideaki; Nakayama, Tomoko; Nishikawa, Yoshifumi; Ikehara, Yuzuru; Kojima, Naoya; 横山, 直明; 井上, 昇; 西川, 義文.
Palavras-chave: Adjuvant; African trypanosome; Immunization; Oligomannose-coated liposome(OML).
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2222

Advances in the artificial feeding of Ornithodoros moubata (Acari: Ixodidae) and the follow up of its life cycle after feeding on fetal bovine serum
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Ruheta, Martin R.; Inoue, Noboru; Fujisaki, Kozo.
Ornithodoros moubata was fed artificially through a parafilm membrane with two types of artificial meals. The first type was composed of bovine and horse red blood cells suspended in phosphate buffered saline or fetal bovine serum (FBS). The second type was composed of FBS alone. Engorgement and post engorgement survival rates of the ticks after feeding on the two types of meals were compared and analysed statistically. FBS was found to be superior to red blood cell meals. Eventually two batches of FBS from different manufacturers were used to feed the ticks through artificial membrane. Engorgement and post engorgement survival rates were again compared and analysed statistically. There was no significant difference between the two lots. Finally O. moubata...
Palavras-chave: Ornithodoros moubata; Fetal bovine serum; Bovine red blood cells; Horse red blood cells; Artificial membrane feeding.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/152

Babesia rodhaini and Babesia microti : Cross-Immunity and Cross-Antigens
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Inoue, Noboru; Omata, Yoshitaka; Igarashi, Ikuo; Saito, Atsushi; Claveria, Florencia G.; Suzuki, Naoyoshi.
Palavras-chave: Babesia; Rodent; Cross-immunity; Cross-reactive antigens.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/210

Characterization of an epimastigote-stage-specific hemoglobin receptor of Trypanosoma congolense
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Yamasaki, Shino; Suganuma, Keisuke; Yamagishi, Junya; Asada, Masahito; Yokoyama, Naoaki; Kawazu, Shin-ichiro; Inoue, Noboru.
Background: Since Trypanosorna spp. lack a complete heme synthesis pathway, the parasites are totally dependent on their host for heme throughout all of the stages of their life -cycle. We herein report the identification and characterization of a T. congolense epimastigote form (EMF)-specific hemoglobin (Hb) receptor. The gene was initially reported to encode a T. congolense haptoglobin (Hp)-Hb complex receptor (TcHpHbR) based on its similarity to a gene encoding a T brucei Hp-Hb complex receptor (TbHpHbR). Methods: Trypanosorna congolense IL3000 was used in this study. A TcHpHbR gene was PCR amplified from the parasite genome. The recombinant protein was used as an immunogen to raise antibodies for immunofluorescence assay and immunoblotting. Hemoglobin...
Palavras-chave: Trypanosome congolense; Epimastigote; Hemoglobin receptor.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4441

CLONING AND EXPRESSION OF AN ANTIGEN OF BABESIA GIBSONI IN ESCHERICHIA COLI AND ITS USE FOR THE IMMUNODIAGNOSIS OF CANINE BABESIOSIS
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志.
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624

Comparison of PCR with Parasitology and Serology in the Diagnosis of a Low Virulent Strain of Trypanosoma brucei gambiense in mice
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Mbati, P. A.; Hirumi, K.; Inoue, Noboru; Situakibanza, N. H.; Hirumi, Hiroyuki; 井上, 昇.
Palavras-chave: Trypanosoma brucei gambiense; Mice; Mel-B; Diagnosis; PCR.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/310

Continuous in vitro Cultivation of Babesia ovata
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Igarashi, Ikuo; Avarzed, Avgaandorjiin; Tanaka, Tetsuya; Inoue, Noboru; Ito, Masanari; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi.
Palavras-chave: Babesia ovata; In vitro culture; Low oxygen atmosphere; Cryopreservation.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/212

Detection of Collagen-cross Reactive Antigenic Components in Toxoplasma gondii
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Omata, Yoshitaka; Inoue, Noboru; Yonematsu, Kunio; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男.
Palavras-chave: Collagen; Cross-reactive antigens; Toxoplasma gondii.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/223

Development of Monoclonal Antibodies That Target 1-Cys Peroxiredoxin and Differentiate Plasmodium falciparum from P. vivax and P. knowlesi
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Hakimi, Hassan; Nguyen, Thu-Thuy; Suganuma, Keisuke; Masuda-Suganuma, Hirono; Ma. M. Angeles, Jose; Inoue, Noboru; Kawazu, Shin-ichiro; 井上, 昇; 河津, 信一郎.
Prompt and accurate diagnosis of malarial patients is a crucial factor in controlling the morbidity and mortality of the disease. Effective treatment decisions require a correct diagnosis among mixed-species malarial patients. Differential diagnosis is particularly important in cases of Plasmodium vivax, a species that shares endemicity with P. falciparum in most endemic areas. Moreover, it is difficult to identify P. knowlesi on the basis of morphology alone, and rapid diagnostic tests are still not available for this malaria species. Therefore, the development of diagnostic tests applicable to the field is urgently needed. 1-Cys peroxiredoxin (1-Cys-Prx) in P. falciparum is abundantly expressed in the mature asexual stages, making it a promising...
Palavras-chave: Malaria diagnosis; Monoclonal antibody; Peroxiredoxin; Plasmodium knowlesi; P. vivax.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3859

Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi,  and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction（PCR）method．The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001％. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281

Different Activation of Lymphocytes in Reaction to Babesia microti Infection in iNOS-/- and Wildtype Mice
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Remer, Katharina; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行.
Palavras-chave: Lymphocytes; Babesia microti; Spleen; INOS-/- mice; CD4+ cells; CD8+ cells; IFN-γ; Antibody-titer.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/263

Establishment of an in vitro Transgene Expression System in Epimastigotes of　Trypanosoma congolense
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Sakurai, Tatsuya; Tanaka, Miho; Shin-ichiro, Kawazu; Inoue, Noboru; 井上, 昇.
The epimastigote form (EMF) of Trypanosoma congolense appears in the late tsetse infective stage. Epimastigotes adhere to the tsetse proboscis, proliferate in this region, and differentiate into mammal-infective metacyclic forms (MCFs). This differentiation is called metacyclogenesis and is indispensable for cyclical transmission of the parasite. Although an in vitro culture method reproducing metacyclogenesis was established several decades ago, few genetic tools have been utilized to elucidate the molecular mechanisms underlying T. congolense metacyclogenesis. In this study, we established a transgene expression system in the EMF of T. congolense IL3000; the EMF was successfully cultured and observed to undergo metacyclogenesis in vitro. The newly...
Palavras-chave: Trypanosoma congolense; Epimastigotes; In vitro metacyclogenesis; Transfection; Transgene expression.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2677

Exposure to 50-Hz electric fields on stress response initiated by infection with the protozoan parasite, Toxoplasma gondii, in mice
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Hori, Takuya; Harakawa, Shinji; Inoue, Noboru; Nagasawa, Hideyuki; Kariya, Tatsuya.
The present study was aimed to examine whether a 50-Hz electric field (EF) affects biological stress responses. Male C57BL/6J mice were exposed to either 50-Hz EF (45 kV/m) or given a sham exposure for 30 minutes per day for 1, 2 or 4 weeks and then either mock inoculated or inoculated with the protozoan parasite, Toxoplasma gondii (T. gondii) PLK strain. Survival rate was calculated in mice infected with T. gondii after exposure to EF and the serum antibody levels to heat shock protein 60 (Hsp-60), and mRNA levels for Hsp-60, IFN-γ and IL-12p35 in mouse peripheral leucocytes were determined. As a result, no statistically significant difference was found in survival rate between mice exposed to EF and those in the sham exposure group. When mice were...
Palavras-chave: 50-Hz; Electric field; Hsp-60; Stress response; Survival rate.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/997

Isolation, cultivation and molecular characterization of a new Trypanosoma equiperdum strain in Mongolia
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Suganuma, Keisuke; Narantsatsral, Sandagdorj; Battur, Banzragch; Yamasaki, Shino; Otgonsuren, Davaajav; Musinguzi, Simon Peter; Davaasuren, Batdorj; Battsetseg, Badgar; Inoue, Noboru.
Background: Trypanosoma equiperdum causes dourine via sexual transmission in Equidae. T. equiperdum is classified under the subgenus Trypanozoon along with the T. brucei sspp. and T. evansi; however, the species classification of Trypanozoon remains a controversial topic due to the limited number of T. equiperdum reference strains. In addition, it is possible that some were misclassified T. evansi strains. Thus, there is a strong need for a new T. equiperdum strain directly isolated from the genital mucosa of a horse with a clinically-and parasitologically-confirmed dourine infection. Methods: Trypanosomes isolated from the urethral tract of a stallion with suspected dourine, were directly cultivated using soft agarose media at 37 degrees C in 5 % CO2. For...
Palavras-chave: Dourine; In vitro culture; Maxicircle DNA; Mongolia; Soft agarose media; Trypanosoma equiperdum.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4442

Kinetoplast DNA and Procyclic Acidic Repetitive Protein A-α Gene of Trypanosoma evansi
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Inoue, Noboru; Honzako, Y.; Hirumi, K.; Xuan, Xuenan; Agatsuma, T.; Nagasawa, Hideyuki; Mikami, Takeshi; Hirumi, Hiroyuki.
Palavras-chave: PARP; KDNA; Trypanosoma evansi; T. brucei.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/276

Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Thekisoe, Oriel M.M.; Rambritch, Natasha E.; Nakao, Ryo; Bazie, Raoul S.; Mbati, Peter; Namangala, Boniface; Malele, Imna; Skilton, Robert A.; Jongejan, Frans; Sugimoto, Chihiro; Kawazu, Shin-Ichiro; Inoue, Noboru.
We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1 fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South...
Palavras-chave: Theileria parva; LAMP; Buffalo; Cattle; Diagnosis.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2672

Mycophenolic acid and its derivatives as potential chemotherapeutic agents targeting inosine monophosphate dehydrogenase in Trypanosoma congolense
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Suganuma, Keisuke; Sarwono, Albertus Eka Yudistira; Mitsuhashi, Shinya; Jąkalski, Marcin; Okada, Tadashi; Nthatisi, Molefe; Yamagishi, Junya; Ubukata, Makoto; Inoue, Noboru.
This study aimed to evaluate the trypanocidal activity of mycophenolic acid (MPA) and its derivatives for Trypanosoma congolense. The proliferation of T. congolense was completely inhibited by adding &lt;1 mu M MPA and its derivatives. In addition, the IMP dehydrogenase in T. congolense was molecularly characterized as the target of these compounds. The results suggest that MPA and its derivatives have the potential to be new candidates as novel trypanocidal drugs.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4492

Non-invasive Method of Identification of SAG-1 Transgenic Mice by PCR Analysis of Oral Wash Cells
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Suzuki, R.; Kato, Mihoko; Bray, R. L.; Lim, C.; Zayatiin, Batsukh; Kamada, Takenori; Inoue, Noboru; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 玄, 学南.
Palavras-chave: SAG-1; Oral wash and two-step polymerase chain reaction.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/311

Preliminary evaluation of oligomannose-coated liposome vaccines against lethal protozoan infections in mice
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Kuboki, Noritaka; Tiwananthagorn, Weerawan; Takagi, Hideaki; Nakayama, Tomoko; Xuan, Xuenan; Inoue, Noboru; Igarashi, Ikuo; Tsujimura, Kunio; Ikehara, Yuzuru; Kojima, Naoya; Yokoyama, Naoaki.
The oligomannose-coated liposome (OML) vaccine is known to induce cellular immunity specific for the encapsulated antigen in immunized mice. In the present study, we preliminarily evaluated the effect of the OML vaccine encapsulating the soluble protozoan lysate of Toxoplasma gondii, Trypanosoma brucei gambiense, or Babesia rodhaini on the corresponding protozoan infections in mice. After the challenge of T. gondii, the OML vaccine group avoided the high mortality resulting from acute infection that was dominantly observed in other control groups. During the infectious course, the development of the T. gondii-specific antibody, which is an indicator of humoral immunity, was constantly controlled at a lower level in the surviving mice of the OML vaccine...
Palavras-chave: Oligomannose-coated liposome (OML); OML; Soluble protozoan antigen; Antibody responses.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1050

Preliminary studies on the effects of orally-administered Transforming Growth Factor-beta on protozoan diseases in mice
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Namangala, Boniface; Inoue, Noboru; Sugimoto, Chihiro; 井上, 昇.
Transforming growth factor beta-1 (TGF-β1) is a pleiotropic cytokine with both pro- and antiinflammatory properties, depending on its environment and concentration. The present study evaluated the effects of orally-delivered TGF-β1 on mice parenterally-infected with various protozoan parasites. We report that while orally-administered TGF-β1 seems to confer partial protection against murine chronic babesiosis and acute trypanosomosis, no beneficial clinical effects were observed against acute babesiosis, malaria or toxoplasmosis. Taken together, these preliminary data suggest that the systemic effects conferred by exogenous TGF-β1 could be parasite speciesspecific. The variations in different parasitic infections could be due to (i) intrinsic differences...
Palavras-chave: Low-dose; Mice; Orally-delivered TGF-β1; Protection; Protozoan parasites.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2716

Production of Transgenic Mice Carrying p30 Gene Encoding Major Surface Antigen of Toxoplasma gondii
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Inoue, Noboru; Igarashi, Ikuo; Nagasawa, Hideyuki; Horiuchi, Motohiro; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 五十嵐, 郁男; 長澤, 秀行.
Palavras-chave: Toxoplasma gondii; SAG-1 (p30); Transgenic mice.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/254

Role of heat shock proteins in protective immunity against infection with Toxoplasma Gondii
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Suzuki, Naoyoshi; Nagasawa, Hideyuki; Hisaeda, Hajime; Igarashi, Ikuo; Horiuchi, Motohiro; Inoue, Noboru; Himeno, Kunisuke.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1061

Seroprevalence of Babesia bovis, B-bigemina, Trypanosoma evansi, and Anaplasma marginale antibodies in cattle in southern Egypt
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Fereig, Ragab M.; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; AbouLaila, Mahmoud Rezk; Guswanto, Azirwan; Thu-Thuy Nguyen; Mohamed, Adel Elsayed Ahmed; Inoue, Noboru; Igarashi, Ikuo; Nishikawa, Yoshifumi.
Babesia bovis, B. bigemina, Trypanosoma evansi, and Anaplasma marginate infections cause serious diseases in cattle, and are primarily transmitted by arthropod vectors (ticks for B. bovis, B. bigemina, and A. marginate and various types of flies for T. evansi). In the last few years, there have been many reports of a high prevalence of certain protozoan infections in northern Egypt, but no accurate or adequate data are available for the southern regions. Therefore, in this study, we screened for evidence of such diseases in economically important cattle species using serum samples. The seroprevalence of protozoan infections in cattle was determined with enzyme-linked immunosorbent assays using species-specific diagnostic antigens. In a total of 301 cattle...
Palavras-chave: Babesia bovis; Babesia bigemina; Trypanosoma evansi; Anaplasma marginale; Cattle; Egypt.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4579

Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Thekisoe, Oriel M. M.; Kuboki, Noritaka; Nambota, Andrew; Fujisaki, Kozo; Sugimoto, Chihiro; Igarashi, Ikuo; Yasuda, Jun; Inoue, Noboru.
In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection of both animal and human trypanosomosis using primer sets that are designed from 5.8S rRNA-internal transcribed spacer 2 (ITS2) gene for Trypanosoma brucei gambiense, 18S rRNA for both T. congolense and T. cruzi, and VSG RoTat 1.2 for T. evansi. These LAMP primer sets are highly sensitive and are capable of detecting down to 1 fg trypanosomal DNA, which is equivalent to 0.01 trypanosomes. LAMP is a rapid and simple technique since it can be carried out in 1 h and requires only a simple heating device for incubation. Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that...
Palavras-chave: LAMP; Trypanosomosis; Trypanosoma brucei brucei; T. b. rhodesiense; T. b. gambiense; T. congolense; T. cruzi; T. evansi.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1045

The establishment of in?vitro culture and drug screening systems for a newly isolated strain of Trypanosoma equiperdum
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Suganuma, Keisuke; Yamasaki, Shino; Molefe, Nthatisi Innocentia; Musinguzi, Peter Simon; Davaasuren, Batdorj; Mossaad, Ehab; Narantsatsral, Sandagdorj; Battur, Banzragch; Battsetseg, Badgar; Inoue, Noboru.
Dourine is caused by Trypanosoma equiperdum via coitus with an infected horse. Although dourine is distributed in Equidae worldwide and is listed as an internationally important animal disease by the World Organization for Animal Health (OIE), no effective treatment strategies have been established. In addition, there are no reports on drug discovery, because no drug screening system exists for this parasite. A new T.?equiperdum strain was recently isolated from the genital organ of a stallion that showed typical symptoms of dourine. In the present study, we adapted T.?equiperdum IVM-t1 from soft agarose media to HMI-9 liquid media to develop a drug screening assay for T.?equiperdum. An intracellular ATP-based luciferase assay using CellTiter-Glo reagent...
Palavras-chave: Colorimetric assay; Drug screening system; Liquid culture; Luciferase assay; Trypanosoma equiperdum.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4538

The Influence of the regulation of Toxoplasma gondii TgMIC2 transgene on host cell infection
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Buates, S.; Xuan, Xuenan; Igarashi, Makoto; Sugimoto, Chihiro; Inoue, Noboru; 玄, 学南; 五十嵐, 慎; 井上, 昇.
Toxoplasma gondii microneme protein 2 (TgMIC2), an apically stored adhesin, was shown to be a key participant involved in the initial attachment and invasion to a host cell. In this study, we had established the clonal line of T. gondii tachyzoites which over-expressed TgMIC2 using the tetracycline repressor (TetR)-based inducible gene expression system. The TgMIC2 over-expression significantly reduced tachyzoite propagation in vitro (p &lt; 0.002) and significantly reduced the parasite virulence in mice (p = 0.002). The over-expression of exogenous TgMIC2 caused the increase of mRNA expression level of endogenous TgMIC2. Wild type parasites mainly expressed TgMIC2115 protein, the microneme store and the surface form of TgMIC2. Over-expression of TgMIC2...
Palavras-chave: Toxoplasma gondii; TgMIC2; Over-expression.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2236

The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Tuvshintulga, Bumduuren; Sivakumar, Thillaiampalam; Battsetseg, Badgar; Narantsatsaral, Sandag-ochir; Enkhtaivan, Batsaikhan; Battur, Banzragch; Hayashida, Kyoko; Okubo, Kazuhiro; Ishizaki, Takahiro; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki.
Babesia microti is a tick-transmitted zoonotic hemoprotozoan parasite. In the present study, we investigated B. microti infection in questing ticks in Mongolia. A total of 219 questing ticks were collected from three different Mongolian provinces (Bayan-Olgii, Khovsgol, and Selenge). Of these, 63 from Selenge were identified as Ixodes persulcatus, while the remaining 156 (from all three provinces) were identified as Dermacentor nuttalli. When the tick DNA samples were screened using a B. microti-specific nested PCR, 19 (30.2%) of the 63 I. persulcatus ticks were found to be B. microti-positive. The parasite was not detected in D. nuttalli. Subsequently, the 18S rRNA, cox1, and tufA sequences of B. microti were amplified, sequenced, and subjected to...
Palavras-chave: 18S rRNA; B. microti; Cox1; Mongolia; Ticks; TufA.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4554

The Role of Scavenger Receptor Type A (SR-A) during Infection with Babesia microti
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Kamada, Takenori; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Suzuki, Hiroshi; Kodama, Tatsuhiko; Suzuki, Naoyoshi; Toyoda, Yutaka; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行; 鈴木, 宏志.
Palavras-chave: Babesiosis; Protective immune response; Knock-out mouse; SRKO.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/265

Toxoplasma Lysate Antigen (TLA144)-Ig8 Antigenic Component : Binding and Induction of Cytotoxic Cell Activity in Mouse Spleen Cells
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Kato, Mihoko; Maki, Yoshiyuki; Inoue, Noboru; Omata, Yoshitaka; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男.
Palavras-chave: Cytotoxic cell activity; NK cell; LAK cell; Toxoplasma lysate antigen.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/216

Transcriptional analyses of Trypanosoma brucei gambiense from infected mice and in vitro culture
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Kuboki, Noritaka; Kibe, Michael K; Thekisoe, Oriel M. M.; Sugimoto, Chihiro; Inoue, Noboru.
With the hypothesis that African trypanosomes could have in vivo specific genes for adaptation to host’s environment, the present study was conducted by using suppressive subtractive hybridization (SSH) technique to seek the highly expressed genes especially in host. A total of 328 clones from the in vivo SSH library and that of 160 clones from the in vitro SSH library were analyzed in order to determine their expression levels, but none of the above-mentioned genes showed differential expression. This indicates that no trypanosome genes could be differentially expressed either the in vivo or in vitro propagated trypanosomes. Alternatively, there might be limitation for detecting specifically expressed genes in African trypanosomes using this method,...
Palavras-chave: Differential expression; Infection; Trypanosoma brucei.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1052

Trypanosoma vivax is the second leading cause of camel trypanosomosis in Sudan after Trypanosoma evansi
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Mossaad, Ehab; Salim, Bashir; Suganuma, Keisuke; Musinguzi, Peter; Hassan, Mohammed A.; Elamin, E. A.; Mohammed, G. E.; Bakhiet, Amel O.; Xuan, Xuenan; Satti, Rawan A.; Inoue, Noboru.
Background: This study was conducted in response to recurring reports from eastern Sudan of camel trypanosomosis that can no longer be treated by currently available trypanocidal drugs. One hundred and eighty-nine blood samples were obtained from camels in different herds and local markets in the western part of Sudan, and a cross-sectional study was carried out between December 2015 and February 2016 to identify the causative agents and possible circulating genotypes. Results: The prevalence of trypanosomes detected using the conventional parasitological techniques of Giemsa-stained blood smears, wet blood smears and the microhematocrit centrifugation technique (MHCT) was 7% (13/189), 11% (21/189) and 19% (36/189), respectively. However, a multi-species...
Palavras-chave: Dromedary camels; Sudan; Trypanosomosis; Trypanosoma evansi; Trypanosoma vivax.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4467

Type-specific PCR assays for Babesia bovis msa-1 genotypes in Asia: Revisiting the genetic diversity in Sri Lanka, Mongolia, and Vietnam
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Liyanagunawardena, Nilukshi; Sivakumar, Thillaiampalam; Kothalawala, Hemal; Silva, Seekkuge Susil Priyantha; Battsetseg, Badgar; Dinh Thi Bich Lan; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki.
Babesia bovis is the most virulent Babesia organism, resulting in a high mortality rate in cattle. The genetic diversity of B. bovis merozoite surface antigens (MSAs), such as MSA-1, MSA-2b, and MSA-2c, might be linked to altered immune profiles in the host animals. The present study aimed to develop type-specific PCR assays for Asian msa-1 genotypes, thereby re-analyzing the genetic diversity of msa-1 in Sri Lanka, Mongolia, and Vietnam. Specific primers were designed for nine Asian msa-1 genotypes, which had been detected based on the phylogeny constructed using msa-1 gene sequences retrieved from the GenBank database. Specificity of the type-specific PCR assays was confirmed using plasmids containing the inserts of msa-1 gene fragments that represent...
Palavras-chave: Asia; Babesia bovis; Genetic diversity; Msa-1; Type-specific PCR.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4489

トリパノソーマ病特異的診断法の開発および野外材料を用いた実用評価
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 井上, 昇; Inoue, Noboru.
平成13年度～平成15年度科学研究費補助金基盤研究(B)(2)研究成果報告書13556048
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2288

原虫病に対する非侵襲迅速診断装置の開発
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 井上, 昇; Inoue, Noboru.
Palavras-chave: アフリカトリパノソーマ; 簡易診断装置; カーボンナノチューブ.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/883